Sentence examples for detergent screening from inspiring English sources

S.Z. did the protein purification and detergent screening.

Several recent membrane protein crystallisation successes have been obtained by detergent screening [11], [27].

After systematic detergent screening, the zwitterionic detergent fos-choline-14 (FC14) was found to be most effective for solubilization and was subsequently used throughout the entire solubilization and purification.

The use of the Fluorescence-detection Size Exclusion Chromatography (FSEC) provides a rapid and efficient method in detergent screening by assessing monodispersity and stability of the target protein.

To measure the degree of trimer formation after solubilization in detergent, aliquots of resuspended membranes corresponding to 10 ml original culture (0.2% total volume) were used in detergent screening experiments (Fig. 1).

A more comprehensive detergent screen identified CHAPS as a stabilizer of MFG-E8 and allowed purification of a significant portion of non-aggregated, full-length protein.

Moreover, FSEC could be employed to figure out the most stable detergents in initial detergent screen.

Further optimization with additive and detergent screens (Hampton Research) was performed, the final optimized crystal condition was 200 mmol/L ammonium citrate tribasic pH 7.5 and 18% (w/v) PEG3350.

In this detergent screen, very thin needles and bunches of needles were obtained in C12E8 (fig. 2C).

This must be accompanied by systematic detergent screens to select detergents that are suitable for long-term stabilization of functional membrane proteins.

Here, we devise a detergent screen that aims to improve the sample quality for solid state NMR.