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(By comparison, 10 ounces of the detergent Method has been selling for several years cleans only 10 loads).
Cells were harvested by centrifugation, the resulting pellet frozen in liquid nitrogen, and extracted using a CTAB detergent method [43].
We carried out biochemical isolation of lipid rafts using the detergent method [ 25].
Lipid raft and non-raft fractions were isolated using the detergent method and each fraction was analyzed by Western blotting.
(B ) Wild-type or Abca1−/− iBMDM were pretreated with GW3965 (1 µM) overnight, followed by stimulation with LPS (10 ng/ml) for 10 min. Lipid raft and non-raft fractions were isolated using detergent method and each fraction was analyzed by Western blotting.
Similar(55)
Introduced the non-detergent method of isolating lipid raft/caveolae membranes using sodium carbonate.
Both adaptors were distributed predominantly at the plasma membrane in resting cells and co-clustered with other raft-associated proteins; however, they distributed differently in buoyant membranes isolated by either detergent resistance or non-detergent methods, indicating that they might localize to distinct rafts.
We have previously shown, using both detergent and non-detergent methods, that cathepsin B localizes to caveolae-enriched fractions of colon carcinoma and endothelial cells [ 10, 12, 26].
We have examined three methods using density gradient based purification of synaptosomes followed by detergent extraction (Method 1) and the pH based differential extraction of synaptic membranes (Methods 2 and 3).
In addition, attempts were made to refold the protein using the detergent dilution method to minimise protein aggregation/precipitation that can result from other detergent removal methods such as potassium chloride-induced detergent precipitation.
The sensor was calibrated with calf thymus DNA in buffer and with DNA extracted from milk using a commercial kit and the detergent extraction method.
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