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The dialysis can be performed in dialysis bags engrossed in large detergent free buffers (equilibrium dialysis)[17].
The beads were allowed to fall through a column of high purity water until detergent free and the fines had been removed.
Double labeling with anti-NG2 was performed using detergent free buffers.
A three-step discontinuous OptiPrep gradient was prepared by layering 1.5 ml of 30% OptiPrep in detergent free lysis buffer and 500 µl of 5% OptiPrep on top.
Also, the buffer for first and secondary antibody dilution was detergent free.
Finally, we have performed detergent free staining of S2R+ cells expressing each receptor variant and observe robust surface localization.
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Most are detergent-free, meaning money can be saved by avoiding expensive cleaning products.
By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed.
Moreover, SapNPs encircle membrane proteins in a compact way, allowing structural investigations of small membrane proteins in a detergent-free environment using small-angle X-ray scattering.
Detergent-free OMV vaccines retain all LPS, which preserves the native vesicle structure, but result in high toxicity and lower yield.
The results substantiate that OMVs from a strain with both mutations, produced with a detergent-free process provide better vaccine characteristics than the traditional detergent-based approach.
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