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A high-content cell-based assay was developed in a 96-well plate format that detects the expression of myosin heavy chain (MHC) in C2C12 cells.
At the same stage, SAGE detects the expression of mir-29b-2 in heart bulbous cordis.
Unexpectedly, microarrays analysis also detects the expression of δ-OAT in pollen, although ornithine pathway seems not able to compensate P5CS deficiency in pollen from p5cs1 p5cs1/p5cs1.
Lysates from synchronized clones were immunoblotted with an anti-phospho-STAT-1 antibody, which detects the expression of serine phosphorylated STAT-1, and an anti-p21 to detect endogenous p21 expression.
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Semi-quantitative PCR was used first to detect the expression of testis/ovary specifically expressed genes.
The placenta was obtained for detecting the expression of LC3-II.
We further used RT-PCR to detect the expression of TNF-h (Fig. 3B).
Roots were used for detecting the expression of nitrate inducible genes by qPCR.
Successful viral infection was confirmed by detecting the expression of matrix protein 1 (M1) (Supplementary Figure S1j).
Allele-specific real-time PCR was employed to detect the expression of H3mm753.
So we detected the expression of ATG5 and ATG7 through silencing or over-expression of miR-3185 in HTR8 cells.
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