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The anti-Opa1 antiserum detects bands of 80 kDa, 100 kDa and 105 kDa (Figure 1B).
The L6/23 antibody detects bands of the predicted molecular weight in western blots of rat and wildtype mouse brain membrane preparations and detects no bands in blots of brain membrane preparations from BK channel (α subunit) knockout mice (Figure S2A).
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The number of detected bands is shown on densitometry plots.
We detected bands of the desired molecular sizes in the gel electrophoresis and observed the desired structures by AFM analysis.
The seven detected bands correspond well with those detected in the absorption spectrum (Figure1).
However, Raman maps recorded for glial cells (Figure 3H) fail to detect bands corresponding to RNA in the cytoplasm.
The software was used to detect bands by normalising against total intensity data for each lane.
Automatically detected bands (checked manually) were used to create matching profiles for lanes (in relation to each other).
The ssrA probe detected bands only in wild type and smpB deletion strains (Figure 4B; lower panel).
Optical density readings for the detected bands were determined using a computer-assisted densitometry program (ONE-Dscan 2.03, Scanalytics).
Once gels are manually oriented using a few matched protein bands, the software automatically matches all detected bands on comparative gels, and measures band densities.
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