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Peroxide block, blocking, antibody incubation, and secondary detection were performed utilizing UltraVision One Polymer IHC detection systems (Thermo Scientific) in accordance with the manufacturer's instruction.
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Apoptosis detection was performed utilizing the ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Millipore, Billerica, USA).
Chromatographic separation, detection, and quantification were performed utilizing a QTRAP 6500 system (AB Sciex, Foster City, CA, USA) equipped with an IonDrive™ Turbo V electrospray ionization (ESI) source and interfaced with an Agilent 1290 series UHPLC system (Waldbronn, Germany).
Western blot analyses were performed utilizing standard procedures.
Morphological studies were performed utilizing SEM.
Spectral analyses were performed utilizing the XWIN-NMR BRUKER suite.
Serum liver function tests were performed utilizing routine laboratory methods.
Therefore, these experiments were performed utilizing the H3/H4 tetramer.
The procedures were performed utilizing the Harding lateral approach.
One-dimensional Western blot analyses were performed utilizing standard procedures.
Statistical analyses were performed utilizing the Statistical Analysis System SASS, version 8.02) [ 29].
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