The software, which is called VIIRS NOAA GMU Flood Version 1.0 (hereafter referred to as VNG Flood V1.0), consists of a series of algorithms that include water detection, cloud shadow removal, terrain shadow removal, minor flood detection, water fraction retrieval, and floodwater determination.
Glucose, fructose and sucrose were separated by High Performance Liquid Chromatography (HPLC), detected by pulsed amperometric detection (Waters 464; Waters) as described by Albertson and Grof [ 58] and quantified by Empower software http://www.waters.com using standard curves calculated from external standards that were processed for each group of ten experimental samples [ 57].
All compounds were quantified by refractive index detection (Waters, Massachusetts, USA).
Trehalose was quantified by evaporative light scattering detection (Waters, Massachusetts, USA).
The samples were analyzed with HPLC on a Waters 2690 separations module, using an AccQTag column (Waters) and fluorescence detection (Waters detector).
Plasma NE was assayed by high-performance liquid chromatography with electrochemical detection (Waters 460; Waters Corp, Milford, MA, USA) [ 13] as previously described by investigators at our laboratory [ 14].
LNAAs (phenylalanine, tryptophan, tyrosine, valine, leucine, isoleucine, methionine, histidine, threonine, and glutamine) were separated by a single-column gradient lithium cation-exchange high-performance liquid chromatography with fluorescence detection (Waters HPLC system, Milford, MA), by using post-column derivatization with o-phthalaldehyde-mercaptoethanol [ 18].
This could be due to the fact that the frequency of NDPhA detection in water resources and drinking water systems is lower compared to the widely studied NDMA.
Before and during the research period, the reference laboratory participated in an external quality-control scheme for Legionella detection in water (Quality Management Ltd, Lancashire, UK), through a periodic distribution of water samples seeded with unknown Legionella spp. and concentration.
Before and during the research period, the reference laboratory participated in an external quality-control scheme for Legionella spp. detection in water (Quality Management Ltd ,Lancashire, UK), through a periodic distribution of the water samples seeded with unknown Legionella spp. and concentration.
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