Sentence examples for detection was performed utilizing from inspiring English sources
Apoptosis detection was performed utilizing the ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Millipore, Billerica, USA).
Peroxide block, blocking, antibody incubation, and secondary detection were performed utilizing UltraVision One Polymer IHC detection systems (Thermo Scientific) in accordance with the manufacturer's instruction.
Detection of apoptotic cells in situ was performed utilizing TUNEL, as described by Wall et al. [ 72].
Measurement of gene expression was performed utilizing the ABI Prism 7000 Sequence Detection System (Applied Biosystems) as previously described [15].
RT-qPCR analyses using SYBR green was performed utilizing an ABI prism 7000 sequence detection system (Applied Biosystems, Foster City, CA).
Animals were sacrificed and tissues collected and processed as described for immunohistochemistry. TUNEL assay was performed utilizing a kit for in situ detection of apoptosis, Oncogene, (Cambridge, MA), according to manufacturer's instructions.
Immunohistochemical staining for GRB10, MYC, SEMA3D, KRTAP1-1, and MEGF10 was performed utilizing an automated Leica Bond III according to the manufacturer's protocol using the Bond™ Polymer Refine detection system.
CBA was performed utilizing supernatants obtained from in vitro mouse and human studies, BD capture beads for IFNγ were incubated for 1 h at room temperature, followed by a 1 h room temperature incubation of the PE detection beads.
