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The species detection was conducted using either molecular absorption spectrophotometry (HPLC UV) or mass spectrometry with electrospray (ESI-MS/MS) as ionization source or inductively coupled plasma (DF-ICP-MS) by monitoring the S-atom contained in both analytes.
A tandem mass spectrometric detection was conducted using multiple reaction monitoring under positive ionization mode with an electrospray ionization interface.
Detection was conducted using a peroxidase-conjugated anti-mouse IgG (CHEMICON International).
Detection was conducted using the Vector Elite ABC detection kit (Vector Laboratories) with 3,3'-diaminobenzidine tetrahydrochloride as the chromogen.
Genomic CHIKV detection was performed for each pool using TaqMan RT-PCR [22], while Dengue virus genome detection was conducted using a previously described method [23].
Allele detection was conducted using an automated capillary CEQ 2000XL DNA Analysis system (Beckman Coulter; http://www.beckmancoulter.com) and electropherograms were analyzed with the Beckman CEQ 2000 v3.0 software.
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Moreover, gear tooth damage detection is conducted using the beta kurtosis and the continuous wavelet transform based on the overall residual signal.
To evaluate the wireless signal network concepts for subsurface event detection, three experiments of event detection were conducted using real wireless sensor nodes (MICAz), where the example events are water intrusion, relative density change, and relative motion.
As discussed, second-stage detection is conducted using the intermediate coefficient matrix Y obtained in the first stage.
Following incubation, HPV detection is conducted using the RHA kit HPV SPF10-LiPA25, version 1 (Labo Bio-medical Products).
In order to obtain a higher accuracy, quality control and CNV detection optimization was conducted using the PennCNV software when generating CNV calls [ 16].
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