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Medical records of the SICU and the database of the Department of Virology were reviewed to identify all patients who were hospitalized on this SICU during the time period when the cluster occurred and who had HSV-1 detected (by antigen detection, virus isolation or PCR) in respiratory fluids.
While respiratory samples have been the most commonly used samples for virus detection, virus may be more readily detectable in serum earlier in the course of illness and in stool samples later in the course of illness (1, 22 ) (Centers for Disease Control and Prevention [CDC], unpub. data).
Clinical samples were collected in Kenya and southern Somalia, and 27 of 115 (23%) hemorrhagic fever patients tested showed evidence of acute infection with Rift Valley fever (RVF) virus as determined by IgM detection, virus isolation, detection of virus RNA by reverse transcription-polymerase chain reaction (RT-PCR), or immunohistochemistry.
Influenza specimens can now be easily and accurately typed or subtyped as H3, H1, or B using a number of methods, such as immunofluorescence assays for rapid antigen detection, virus culture and subsequent antigenic analysis, and reverse transcription polymerase chain reaction assays [13].
To validate the extraction and/or PCR procedure in the progress of detection, virus lysate and in vitro transcribed RNA were introduce into the panel.
In Italy, swine influenza monitoring programs, based on specific genome detection, virus isolation, and molecular characterization of influenza viruses causing respiratory forms, have been in place since the 1990s.
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aN reflects the total number of participants, reduced by the number of co-detections, viruses with <20 detections, and participants without follow-up surveys.
Virus detection PCR Herpes virus type 1 and 2 (HSV1, HSV 2) and HZV DNA were detected by PCR.
Detection of virus in nasal washes is a key biomarker for influenza virus infection.
Statistical analysis of data showed significant differences between ELISA and RT-PCR for detection of virus in aborted fetuses.
Detection of virus specific IgM in serum is the most commonly used method.
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