Both total RNA and small fragment RNAs can be used for detection, and the amount of the latter needed for detection was only approximately one-quarter of the total RNA.
Five different configurations were characterized using cyclic voltammetry and evaluated in an amperometric sensor setup for nitrite detection where the amount of the different components were optimized.
In contrast, FRET microscopy permits the direct detection of the amount of active RhoA in intact living cells during E7-transformation.
Our use of a threshold means that proportionately more of the smaller mEPSCs in caffeine failed to reach detection reducing the amount of caffeine-mediated inhibition.
Both the frequency of detection and the amount of methylated CAHM DNA released into plasma increased with increasing cancer stage.
This assay is based on the detection of the amount of Ac-DEVD p-NA substrate cleaved by cell lysates to release the colored p-NA molecule.
For instance, using a camera with a filter for excitation (415 nm) and a filter for emission (635 nm) allows detection of the amount of photosensitizer in the tissue.
However, as the determination of the cell cycle profile relies on the detection of the amount of DNA – half the amount of DNA in G1 when compared to G2 phase – it does not allow a distinction between tetraploid cells and G2 cells.
Repartitioning of co-solvents between particles of latex emulsions was investigated by means of a fluorescence method based on the detection of the amount of co-solvent via the solvatochromic shift of the emission maximum of a fluorescent probe, copolymerized at a low concentration.
The single largest barrier to using circulating DNA for cancer detection is the amount of circulating undegraded DNA that can be isolated is low, making it unsuitable for currently available assay technologies.
