Apoptotic cells were detected by in situ cell death fluorescein detection kit (terminal dUTP nick-end labeling [TUNEL]; Boehringer Mannheim) and quantified by flow cytometry.
The in situ cell death detection Kit (terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling, TUNEL) from Roche Diagnostics GmbH (Mannheim, Germany) was used and 3,3'-diaminobenzidine (DAB) was purchased from Sigma (Deisenhofen, Germany).
Apoptosis detection by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) analysis was performed using the Apoptag detection kit (Serologicals Corp., Norcross, GA) according to the manufacturer's instructions.
The high P(N) of coho populations (100% for the Tenderfoot Creek release groups and 84% for the Coldwater River group) are based almost exclusively on fish detections at NSOG without subsequent detection at terminal stations.
Findings consistent with FPHL include the detection of terminal hair loss, variation of hair caliber, and miniaturized hairs.
The cells were then stained with Ki67 or Apoptosis Detection Using Terminal Transferase and Biotin-16-dUTP (TUNEL) to evaluate proliferating and apoptotic cells, respectively.
TUNEL assay was performed according to manufacturer's instructions (In Situ Cell Death Detection Kit, Terminal Red – Roche Applied Science, Indianapolis, IN, USA).
The in situ cell death detection kit (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)) was purchased from Roche Diagnostics (Mannheim, Germany).
In Situ Apoptosis Detection Kit (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, TUNEL) was from Chemicon International (Temecula, CA, USA).
However, some of our findings are in conflict with assumptions about the detection of terminal fusion in this species [ 4, 29, 30], unless this was previously erroneously reported.
In fact, detection of terminals without a visible varicosity or calpain-2 labeling was an exception.
