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Protein signals were detected by electrochemiluminescence detection Quantity One® software (Bio-Rad Laboratories).
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The He-PDPID can detect permanent gases, volatile inorganics and other compounds which give little or no response with the flame ionization detector (FID) and has significantly better limits of detection (minimum detectable quantities (MDQs) in low picogram range) than can be achieved with a thermal conductivity detector (typically not lower than 1 ng).
Although betacellulin and amphiregulin mRNA levels were above the specified limit of detection, the quantities detected by qRT-PCR were very small in the majority of patients.
The detection and quantity of RSV in respiratory specimens was associated with younger age, but not with gender or hospitalization.
Logistic and linear regression models with general estimating equations (GEE) and an exchangeable correlation matrix were used to assess factors associated with detection and quantity of cervico-vaginal HIV-1 RNA, respectively.
Suppressive therapy for herpes simplex virus type 2 (HSV-2) has been shown to reduce both detection and quantity of genital HIV-1 RNA over periods of upto three months in several randomised controlled trials (RCTs) [6], [7], [8], [9], [10], [11].
It results from a nearness to the detection and quantity limits obtained by ED-EPMA.
Detection and quantity of RNA was determined using a Bioanalyzer and RNA 6000 Nano chips according to the manufacturer's protocol (Agilent Technologies, Santa Clara, CA, USA).
While this limited the power of our analyses, the results remain useful, as they demonstrate that there were not large increases in either detection or quantity of vaginal HIV-1 RNA associated with episodes of T. vaginalis infection.
In addition to comparing the gross errors of the algorithms in terms of the fundamental frequency, we also want to measure the detection lag, a quantity that is a fidelity indicator for real-time fundamental frequency tracking.
The observation of a dichotomous oral replication pattern in Ugandans with asymptomatic HHV-8 infection, between those with frequent and high quantity detection or infrequent and low quantity detection, is similar to results seen in a US based population of asymptomatic HHV-8 infected MSM [11], [12].
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