Chromatographic peaks were detected between 0 and 18 min with a retention time error window of 0.1 min. Apex track peak detection parameters were used, automatically detecting peak width and baseline noise.
The number of detected retention time/mass pairs depends on the peak detection parameters applied and varies with varying settings.
By varying the detection parameters of the method, a significant difference in the sequence characteristics was detected between the genes residing inside the clusters and those outside the clusters.
In conclusion, we have presented a simple mathematical model that analytically relates physical detection parameters of a single nanoparticle LSPR sensor to the minimum number of detectable analyte molecules.
Raw electropherograms were analysed with PEAKSCANNER V1.0 (ABI, using default peak detection parameters except a light smoothing and 100 rfu as fluorescence threshold) to detect and calculate the size of AFLP bands.
Detection parameters are selected in the following way.
Important detection parameters, pH and detection temperature were optimized.
Values of all detection parameters are the same as in previous assessments.
After optimization of MS detection parameters, this method has been validated for spiked drinking water samples.
The detection method uses wavelet energy (WE) and zero crossing rate (ZCR) as detection parameters.
The recommendations, however, depend on the quality of the input images and the edge detection parameters.
