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Open image in new window Fig. 4 Detection of toxins.
Aptasensors, as emerging methods, have opened new horizons for sensitive and selective detection of toxins.
Cell-based potentiometric sensor for the detection of toxins was fabricated by attaching endothelial cells to a K+ selective membrane.
The use of nanotechnology in bioanalytical devices has special advantages in the detection of toxins of interest in food safety and environmental applications.
This paper gives an overview of the electrochemical biosensors that have incorporated carbon and metal nanomaterials in their configurations for the detection of toxins.
The expression of voltage dependent sodium channels in HEK cells is anticipated to provide enhanced performance for cell-based detection of toxins for drug and natural product discovery, biomonitoring and environmental monitoring.
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Therefore, development of rapid and sensitive methods for the detection of toxin-producing microalgae is necessary for warning of water quality.
Traditionally, enzymes and antibodies have been integrated in the biosensor designs as a popular choice for the detection of toxin molecules.
Multiple enzyme immunoassays (EIAs) have been introduced by various manufacturers for the detection of toxin A alone or for both toxins A and B. Some of these are designed to give results in less than 1 h.
The occurrence of genes capable of producing toxins in other strains of Bacillus sp. has already prompted the Scientific Committee on Animal Nutrition (SCAN) to recommend that products from Bacillus sp. other than those from B. cereus group should be accepted only if there is no detection of toxin production (SCAN 2000).
The detection of toxin-induced ADP-ribosylation of eEF-2 in intact cells was performed as previously described [18].
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