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For two color in situ hybridization, NBT/BCIP was used to detect the first (and weakest) probe, prior to detection of the second probe with Vector red.
For fluorescence double in situ hybridization, the probes were detected sequentially, and the slides were incubated in 10 mM HCl before detection of the second probe.
The detection of the second signal (SSS) will determine the physical cell ID of the macro cell.
This suggests that the discovery of the first categorical target 'primed' the detection of the second (Kristjansson, Saevarsson, & Driver, 2013; Maljkovic & Nakayama, 1994).
A Nd YAG, (YAG = yttrium aluminum garnet) laser, operating at 1064 nm, was used allowing the detection of the second harmonic at 532 nm (green), in the region of many cameras peak sensitivity.
Following detection of the index case, a broiler breeder farm, a surveillance program was initiated, which led to the detection of the second case on 11th March.
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To investigate the effectiveness of the control measures, A. Stegeman et al. quantified the transmission characteristics of the H7N7 strain before and after detection of the first outbreak of avian influenza in The Netherlands in 2003 [34].
A second malignancy may represent synchronous cancers (Fig. 4) (i.e. cancers detected simultaneously) or metachronous cancers (i.e. cancer with an interval between detection of the first malignancy and detection of a subsequent tumour).
Nicastro, F. et al. Chandra detection of the first X-ray forest along the line of sight to Markarian 421.
Metastasis-free survival (MFS) was determined as the interval between initial diagnosis and detection of the first metastasis.
A decade ago, the detection of the first transiting extrasolar planet provided a direct constraint on its composition and opened the door to spectroscopic investigations of extrasolar planetary atmospheres.
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