For detection of the loading control we used mouse monoclonal anti beta Tubulin (1 1000 dilution, DSHB, Iowa, US) as primary and peroxidase labeled antimouse antibody as secondary antibody (1 10000 dilution, NIF 825, Amersham, GeHealthcare, Freiburg, Germany).
Before detection of the Ca2+ load in ADRP retinas with ongoing UPR, we validated the method of Ca2+ measurement and provided proof of principle that the activated UPR causes the release of the Ca2+ into the cytosol.
The limit of detection of viral load assays used throughout the study period varied from < 400 to < 50 copies/ml.
Quantitative real-time RT-PCR was also used for detection of virus load in the brain, lung, nasal turbinates, ileum, and blood.
Manual QIAmp extraction method with enzyme pre-treatment resulted the most sensitive for detection of bacterial load.
The strategy is based on a novel detection method of the harmonic load current.
However, a small number of LTNPs exhibited suppression of viral replication to levels below the detection threshold of the viral load assay.
Through the detection of axial bolt load in different sections of roadways, the status of real-time bolt support quality can be reflected; meanwhile, the rationality of bolt support design can be evaluated which provides reference for bolting parameters optimization.
Additionally, the use of a turbidimeter allows for quantitative detection of viral load [ 32].
Pre-warning technology of roadway surrounding rock stability is proposed based on the detection of axial bolt load.
