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Detection of the compounds was based on isolation of the protonated molecular ion, [M + H]+, and subsequent MS/MS fragmentations and selected reaction monitoring were performed.
Matrix effect, although present especially for mephedrone, is acceptable, allowing the detection of the compounds at the LODs described.
Detection of the compounds was done by DAD at 200, 224 and 278 nm.
Calibration curves of all analytes routinely yielded correlation coefficients 0.999 or higher, and the detection of the compounds was carried out with a UV-detector at 280 nm.
A photodiode array detector provided simultaneous detection of the compounds as follows: amygdalin (215 nm), paeoniflorin (232 nm), cinnamaldehyde (280 nm), and paeonol (275 nm).
Calibration curves of all analytes routinely yielded correlation coefficients 0.999 or better, and the detection of the compounds was carried out with a UV-detector at 280 nm.
Similar(50)
Aliquots were removed at distinct time points as indicated and analyzed by LC-MS/MS with detection of the compound at 254 nm.
However, the environmental sampling was focused on the qualitative detection of the compound.
The concentration of the compound in urine was below the LOD (about 0.1 ng/ml), despite detection of the compound in whole blood specimens.
The voltammetric studies using the thionine nafion modified electrode show two well-resolved anodic peaks for AA and UA, revealing the possibility of the simultaneous electrochemical detection of these compounds in the presence of biological thiols.
Another factor in method development and application was the large amount of litigation that occurred because of the detection of these compounds in humans as well as the environment.
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