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Although further studies testing sera from a higher number of chagasic patients from endemic areas could be necessary to draw conclusive data for an immunological clinical follow-up, the results presented here show that the detection of reactivity against different and specific antigens may be useful for monitoring the effectiveness of benznidazole treatment.
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Our results showed that serum factor protein microarrays facilitate detection of autoantibody reactivity to serum factors in human samples and that BAFF-reactive autoantibodies may be associated with an elevated inflammatory disease state within the spectrum of SLE.
Detection of antibody reactivity was performed using Immobilon Western HRP Substrate (Millipore).
The presented principle is applied for the detection of antibody reactivity against UH-RA.21 which is a good candidate biomarker for rheumatoid arthritis (RA).
Success of any eye stimulation technique or imaging modality is determined by its practicality for clinical use and its reliable detection of vascular reactivity response.
Indeed, detection of macroschizont reactivity by an anti-SVSP serum was limited to a small number of cells and endogenous polypeptide was not detected by immunoblotting [ 20].
Finally, our pools of sera were from three patients each because this number was sufficiently low to allow the detection of strong reactivity that would be present in the serum of a single individual [ 25].
Detection of IgG reactivity was carried out as previously reported [ 22- 24] (Additional file 1) with the use of a γ-chain-specific secondary rabbit anti-human IgG antibody coupled to alkaline phosphatase.
For most antigens in this study, detection of autoimmune reactivity has been previously verified in earlier reports using appropriate patient groups in which (a) the target antigen or autoantigens are known and (b) the cohort contains patients positive for the clinical diagnosis and positive for the target antibodies in their serum.
In our study, sera from rabbits with common parasitic infections were selected as control groups for the detection of cross-reactivity.
Serum samples infested with Psoroptes cuniculi (n = 12), Eimeria stiedae (n = 6) and Taenia pisiformis (n = 6) were used as controls for the detection of cross-reactivity.
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