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Fluorescence in situ hybridizations for the detection of losses in 13q14 and 17p13, gains in 1q21, 9q34 and chr11, and for the translocations (4 14), 14 16) and (11 14) were performed according to standard protocols (Supplementary Table S1).
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L0 and G0 were scored if there was no detection of loss or gain of somatosensory function respectively [19].
MAPH can provide extremely high resolution and enable the sensitive detection of loss or gain of genomic DNA sequences as small as 150 bp.
This clinical report underscores the need to check vital signs carefully to ensure early detection of loss of consciousness that could lead to PRES, even when there is no history of hypertension.
Optical measurements allow visualization of wave propagation and resonance patterns in SAW devices as well as the detection of loss sources and undesired responses, such as escaping acoustic beams, unwanted reflections and acoustic crosstalk.
This FISH strategy permits the detection of loss and gain of specific regions.
Detection of loss of heterozygosity (LOH) is usually performed on homogenised tumour specimens.
Allelotypic detection of loss of heterozygosity (LOH) has been used to identify putative tumour-suppressor genes.
At the same time, we wanted to define the critical timing and number of morphological features that quantitatively allows detection of loss of hBMSC differentiation potential.
We describe our methodology, and discuss its application to other types of paired-tissue analysis such as the detection of loss of heterozygosity as well as allelic imbalance.
Taq DNA polymerase can introduce sequence errors during PCR amplification, and unequal amplification of the two alleles can result in false positive detection of loss of heterozygosity.
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