Sentence examples for detection of insertion from inspiring English sources

The software performs three steps: (i) construction of the de Bruijn graph of the reads, (ii) detection of insertion breakpoints on the reference genome (find module) and (iii) local assembly of inserted sequences (fill module).

This includes the use of flow cytometry (for ploidy analysis), diagnostic PCRs (to confirm completeness of the insert), qRT-PCR (for gene expression analysis) and southern blotting (for detection of insertion number).

This hybrid method facilitated detection of insertion mutations and allowed annotation from MG1655.

All the methods are based on detection of insertion sequence IS 6110.

Nevertheless, the significantly enhanced detection of insertion events can definitely improve interpretation largely as they may cause impactful disruption in the genome.

The additional development of a PCR-based screening strategy permitted the rapid detection of insertion mutants in any chromosomal region of interest.

Polymerase chain reaction detection of insertion-deletion polymorphism was used to determine the presence of the two angiotensin-converting enzyme alleles in the groups; the frequencies in the general population in our area are presented for comparison.

Detection of insertions by 'galign' follows similar considerations to deletion detection, however, unlike deletions, whose size can be estimated, insertion size is either difficult or impossible to extract from the 'galign' alignment output (although with the incorporation of lesion-spanning reads in the upcoming 'galign' release, sizes of small insertions will be measurable).

The splinkerette approach produced PCR fragments of defined size and some genomic sequences, allowing the estimation of the region of the insertion in some mutants and detection of insertion-chromosome junction in others (Additional file 2: Table S1).

Suppression of marker gene expression makes detection of insertions in such regions nearly impossible.

Detection of insertions might be improved by manipulation of the hybridization/wash conditions or changes to the algorithm to specifically identify probe series indicative of insertions.