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The detection of CAPS polymorphisms and design of primers was separated into two tools, which were modified to use Galaxy interval format and GFF3/GVF formats [ 35] as the input and output formats.
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A minimum required performance limit (MRPL) for analytical methods of 0.3 μg kg−1 was set by the European Community for the detection of CAP residues in food matrices.
Consequently, the fluorescence intensity recovers and the recovery extent can be used for detection of CAP in homogenous phase via optical responses.
Our approach, based on detection of a multiparametric expression signature, yielded the best performance at detection of CaP ever published with single predictors as far as we know.
Although detection of CaP at early curable stage is highly desirable, actual screening methods present limitations and new molecular approaches are needed.
Since prognosis is generally unfavourable when disease is no longer organ-confined, detection of CaP at an early curable stage is highly desirable, but unfortunately available methods such as serum Prostate Specific Antigen (PSA) present limitations [1].
The early detection of CaP is actually carried out by the association of digital rectal examination (DRE) and serum total prostate-specific antigen (tPSA) level.
Animals grazing on pasture where such herbaceous plants are prevalent or being fed with feedstuffs containing those plants may become contaminated with CAP, with the subsequent detection of CAP in the animal products.
Although these tumour suppressor genes are unknown, we have previously shown accurate detection of CaP by investigating genetic alterations in prostatic cells collected in urinary sediments (Cussenot et al, 2001).
This paper describes a method to reveal the illegal use of chloramphenicol (CAP) in animals intended for human consumption based on the detection of free CAP and chloramphenicol-glucuronide (CAP-glu) in urine.
The replication defect results from reduced protein synthesis as well as from detection of incompletely capped RNAs by cellular innate immunity sensors.
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