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Fig. 2 The working principle of the α-HL nanopore-based detection of bacteria at single-particle level.

The more complete protocol with command used is outlined in Additional file 3. The detection of bacteria at various phylogenetic levels is often required during medical diagnosis and in both epidemiological and ecological studies.

Samples for detection of bacteria in blood were obtained at baseline (before surgery) and at death using routine sampling and assays according to the procedures at the hospital's intensive care unit.

The detection of bacteria during the early stage of an infection can prevent severe human disease.

In order to assist detection of bacteria in infected macrophages, strains were transformed with a GFP-expressing plasmid (pFVp25.1).

This novel method has a great potential application in rapid detection of bacteria in the near future.

Yamamoto, Y. PCR in diagnosis of infection: detection of bacteria in cerebrospinal fluids.

Detection of bacteria was performed at each hospital laboratory.

This system has potential application in the detection of bacteria concentration of milk at dairy farms.

Subcultures of Tarozzi bouillon followed after 24 h incubation at 37°C to augment detection of bacteria.

In conclusion, we found that qPCR is a reproducible method for detection of bacteria including anaerobic bacteria from CF airway samples.

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