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A first detection with sensor 1 occurs, but is not yet confirmed by sensor 2. The second detection is confirmed by sensor 2 which sends an alarm to the supervisor.
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The specificity of the PCR detection was confirmed by sequencing analysis of the detected mRNA-derived PCR products; the detected DNA sequences of the PCR products were 95%to100%0% identical to reference sequences in Genbank (data not shown).
Hypothesis 1 concerning fault detection was confirmed by the experiments.
The specificity of the Neuro-2a cell-based assay for palytoxin detection was confirmed by using ouabain, which also acts on Na+, K+-ATPase.
Emory and Nie investigated a near-field microscopic system to employ SERS on a single silver nanoparticle, and a highly improved sensitivity of molecular detection was confirmed by the measurements of Rhodamine 6G and 3-hydroxykynurenine attached onto the silver nanoparticles [64] in Fig. 2a.
Selective detection was confirmed in excised tissues by in situ imaging and histology (Figure S2 and S4).
16S rDNA sequence-based detection was confirmed in all specimens by further amplification and sequencing of specific rpoB gene fragment (Table 1).
In all instances, negative controls remained negative and the 16S rDNA based detection was confirmed by a second rpoB gene-based amplification and sequencing of specific sequence.
In the present study, the specificity of M. smithii and M. stadtmanae detection was confirmed by evaluating two independent, universal molecular targets (16S rRNA and rpoB genes) using specific and highly sensitive quantitative real-time PCR.
Specificity of detection was confirmed using no probe staining and RNase-digested specimen.
Specificity of α-gal detection was confirmed by its enzymatic removal using α-galactosidase.
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