Then, the same stool samples for norovirus detection were frozen and stored in <20°C for future simultaneous testing with the IDEA™Norovirus (DakoCytomation; Oxoid, Ltd) according to the manufacturer's instruction.
Because the blood samples were transported frozen, detection of morulae was not possible.
Next we investigated the reproducibility of single nucleotide variation (SNV) detection in snap frozen versus FFPE tissues.
However, clinical utility of these molecules as prognostic markers is limited by the use of ELISA tests for their detection in fresh or frozen tissue [ 46].
For the detection of neutral lipids, frozen sections were sliced and stained with the oil red O, using 0.5% oil red O dissolved in propylene glycol for 10 min at 60°C.
For chromogenic detection of SeAP activity, frozen sections were first incubated at 65°C in PBS for 30 minutes to inactivate endogenous alkaline phosphatase activity and were subsequently incubated with NBT/BCIP (nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate; Roche Diagnostic, Indianapolis, IN) in 0.1 M Tris-HCl, pH 9.5, 0.1 M NaCl and 50 mM MgCl2 overnight at room temperature.
Data collected from all articles included title, authors, journal, year published, country, description of patients, anatomical sites of cancers analyzed, type of sample (formalin-fixed, paraffin-embedded versus fresh frozen), HPV detection method, sample collection period, overall number of head and neck cancers analyzed, and number of cancers that were HPV+.
Finally, the system for SAEC must include a double-talk detection module which "freezes" the adaptation procedure whenever a near-end signal is detected in order to avoid the divergence of the adaptive algorithm [18, 19].
At day 3, 7, 14, and 28 after injections, animals were sacrificed and organs were carefully collected and frozen for plasmid detection.
