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This early implementation of an APS-based direct detection detector (DDD) has already delivered unprecedented performance in many areas exceeding the fundamental capabilities of CCD-based systems.
The phenols were identified by their retention times and UV absorption spectra between 230 and 370 nm, recorded continuously by the diode array detection detector, and the extinction at 280 nm was used for quantification.
This article highlights the most important developments in flow analysis with chemiluminescence (CL) detection, describing different flow systems that are compatible with CL detection, detector designs, commonly applied CL reactions and approaches to sample treatment.
The neutral sugars during saccharification process were analyzed by HPLC (Waters 2695e, USA) with Aminex HPX-87P (300×7.8 mm, Bio-Rad, USA) at 85°C and refractive index detection detector at 35°C.
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Next, ( {q}_{l_1,{l}_2} ) is converted back to the electrical domain by the direct detection detectors.
Scenario 2: two detectors, independent filters (2d in f): The separation is improved by using polarized excitation and polarization-resolved detection; one detector for the parallel and one for the perpendicular component (see Figures 1 B and 9 C, D).
The integration of this electronics in custom application specific integrated circuits (ASICs) is mandatory when the compactness of the detection system (detector and electronics) and the need to read out multi-elements SDDs are primary requirements.
For the pure UCNC luminescence detection, the detector slit size was 0.5 um and spectrum integration time is 0.5 s.
When IMS is coupled with MS, ions are separated prior to detection, reducing detector suppression while supplying an additional dimension for peptide identification.
The samples were analyzed with HPLC on a Waters 2690 separations module, using an AccQTag column (Waters) and fluorescence detection (Waters detector).
PAHs were determined by a high performance liquid chromatography (HPLC system Agilent 1100 Series-thermostatted autosampler G1329A, binary pump G1312A) with fluorimetric detection (fluorescence detector G1321A by Agilent Technologies) (HPLC-FLD).
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