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Analysis of population dynamics allows for distinguishing between the rate of detection and variation in the infected population size and presents an important tool for the identification of emerging diseases.
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A first selection based on the detection call and variation coefficient of all the CC samples from aged and young patients delineated 9,802 transcripts.
Well-documented examples include antigenic variation in parasites and fungi to evade adaptive immune responses, viral evasion of immune detection, and phase variation in bacterial pathogens [ 1- 5].
Over the years, polymerase chain reaction (PCR) has evolved beyond simple amplification and detection, and several variations have been described.
Figure 8 Local edge detection and maximum gradient variation.
DNA arrays are potentially powerful experimental tools within neuroscience but application of this technology to in vivo paradigms may, in practice, be limited by the sensitivity of transcript detection and inter-screen variation.
Levels of detection and coefficients of variation have been described in detail elsewhere [ 1].
Processing of Illumina reads, mapping and variation detection was performed using the CLC Genomics Workbench 5 (version 5.5.1).
PCR duplication reads during sequencing, which affect the sequencing depth and variation detection, were excluded by an in-house script.
We investigated different combinations of the input sequence format and variation detection modules and found that potential varietal SNPs between two tetraploid cotton varieties could be quite accurately called using a nonredundant read set and the "quality-based variation detection" module by following the criteria detailed in the section Materials and Methods.
These results show the possibility to process WGA gDNA samples at nearly similar performance to that of standard non-amplified gDNA samples without showing significant allelic bias/difference in enrichment metrics and variation detection among non-pooled and pooled samples.
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