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The traditional means for detection and speciation of mollicutes from clinical samples have been culture and serology.
By using two sets of PCR primers, one for a universal bacterial gene (16S rRNA) and one for a species-specific gene (nuc), the molecular protocol allowed both the detection and speciation of the bacterial infection.
Polymerase chain reaction with specific primers will allow the detection and speciation of microsporidia in biopsy tissue, bodily fluids, and stool specimens.
A nested-PCR offers increased sensitivity over a primary PCR and should be evaluated with currently used methods for the routine detection and speciation of Bartonella henselae and B. clarridgeiae.
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Configurations of the basic sequential injection speciation analysis systems were designed around a multi-syringe-time-based-injection system with one detector, direct and indirect speciation of different forms using a single detector including diode array detection and direct speciation of different forms using multiple detection.
Detection of fungi and speciation of isolates were performed at the laboratories of participating hospitals, in accordance with their standard protocols.
The approach used in the detection of genome duplication and speciation event was adapted from Blanc and Wolfe.
Local collaboration, early detection, and diagnosis, along with speciation of the parasite, were of paramount importance to ensure the effective delivery of treatment and successful control of the epidemic.
However, despite the relative importance of pheromone detection in the evolution of insect communication and speciation, the genetic mechanisms underlying the differentiation of pheromone recognition systems remain poorly understood [ 7, 8].
Furthermore, its role in pheromone detection may contribute to evolutionary processes, such as reproductive isolation and speciation.
From the examples of Ircinia and Psammocinia we demonstrate that barcoding facilitates rapid assessment of biodiversity, radiation patterns and the detection of cryptic speciation.
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