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They cannot only be used for affinity purification but also downstream for detection and assay of an arbitrary fused recombinant protein without the need for any prior knowledge of its biochemical properties.
Non-destructive detection and assay of nuclear materials is one of the most critical issues for both the management of nuclear waste and the non-proliferation of nuclear materials.
Detection and assay of SNPs are amenable to automation and thus useful for high-throughput genotyping [ 1].
ELISA methods rely on antibodies for protein detection and assay development ideally uses two antibodies against different epitopes of the candidate TBI marker.
They have been widely utilized, for instance, in the detection and assay of proteins,[ 4] antibodies,[ 5] and nucleic acids.[ 6] In the case of nucleic acids, DNA-templated catalytic processes in particular have been successful.
Along with parameters commonly provided by manufacturers (e.g., assay sensitivity, lower limit of detection, and assay precision determined by reproducibility of replicate measurements), immunoassay utility should be evaluated also by matrix-specific fold change in cytokine concentration reliably detectable by multiple comparisons methods.
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The introduction in the 1930s of microbiological assay methods that used lactic acid bacteria for the detection and assaying of vitamin factors greatly aided in the discovery, isolation, and characterization of many B vitamins.
In conclusion, we established a real-time RT-PCR assay for CxFV detection, and this assay is more sensitive and efficient than general RT-PCR.
As the format, signal detection system, and assay standardization of these two ELISA systems were different in many ways.
For years, the Farr technique has represented the method of choice for anti-dsDNA detection and quantitative assay.
In a prospective case-control study, we studied 78 patients with MS and 123 healthy controls for viral DNA detection and antibody assay.
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