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Unoxidized and oxidized phosphatidylcholine (PC) and phosphatidylinositol (PI) were measured by normal phase high-performance liquid chromatography with diode array detection, and analyzed with a general linear model for repeated measures analysis of variance.
Samples were then fixed with 2% paraformaldehyde, and CPMV identified through fluorescent antibody detection and analyzed by flow cytometry.
Blots were imaged by a Typhoon phosphor-imager (GE Healthcare, UK) using enhanced chemi-fluorescence detection and analyzed using ImageQuant software (GE Healthcare, UK).
To investigate the mechanism of Ad.IL3-gene-induced cytotoxicity of leukemia cells, HL60 and KG-1 were treated with PBS, Ad.IL3, Ad.IL3-PPA or Ad.IL3-MnSOD, followed by staining with Annexin V-FITC and propidium iodide (PI), a common method for apoptotic cell detection, and analyzed under a flow cytometer.
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In this framework, performing community detection and analyzing the cluster evolution represents a critical task.
Fluorescence was detected with the ABI Prism 7900HT Sequence Detection System and analyzed with ABI Prism Sequence Detection System software (version 2.0; Applied Biosystems).
A planar micro-machined tri-axis gyroscope in decoupled-mode mechanical structure is presented, verified for superior detection capability and analyzed by experiments in this paper.
Beads were washed and incubated with the phospho-antibodies and detection reagents and analyzed with a Bio-Plex 200 System (BioRad) instrument.
Determination of copy number variation by quantitative real time PCR was performed using the Applied Biosystems 7900HT Sequence Detection System and analyzed with the SDS 2.2 software following the guidelines of the manufacturer (Applied Biosystems).
Real-time analysis of PCR amplification was performed on an Applied Biosystems 7900HT Sequence Detection System and analyzed with an SDS 2.3 Software Applied BiosystemsSDS 2.3 Software Applied Biosystems
Real time PCR was performed in a CFX96 Touch™ Real-Time PCR Detection System and analyzed with the CFX Manager™ Software (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
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