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Edge detecting was conducted using a canny edge detector.
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ELISA to detect insulin was conducted using Millipore Human Insulin ELISA kit.
If a significant difference in any factor and/or interaction term was detected, post hoc analysis was conducted using Tukey's HSD.
Locations of aberrations specifically observed in the p62 transgenic animals were detected using the Golden Helix software: analysis was conducted using SNP an Variation Suite v8.
A similar comparison between the N. vectensis genome assembly and the A. millepora sequences was conducted using tblastx, to detect any coral sequences with anemone orthologs that had been missed by gene prediction algorithms.
The reaction yield was measured by detecting the concentration of unreacted Au3+ via ICP-MS, which was conducted using an ELAN 6100 instrument (PerkinElmer SCIEX, Waltham, MA, USA).
This study was conducted using the PAM-RL ambulatory device to detect periodic leg movements.
A check for stakeholder bias was conducted using the coding summaries, but no apparent bias was detected.
To detect the SNPs and InDels of Ilpumbyeo and Moroberekan, resequencing with NGS (Next-Generation Sequencing) was conducted using the two parents according to Jeong et al. ([2013]).
The QTL analysis was conducted using the SARS program, and three QTLs for natural spikelet fertility were detected (Table 3).
Co-IP of HA-tagged proteins was conducted using anti-HA antibody and presence of proteasome in the precipitates was detected using anti-S2 and anti-S5a antibodies.
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