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Roots were used for detecting the expression of nitrate inducible genes by qPCR.
The placenta was obtained for detecting the expression of LC3-II.
Successful viral infection was confirmed by detecting the expression of matrix protein 1 (M1) (Supplementary Figure S1j).
Redox balance was evaluated by detecting the expression of antioxidant defenses and ROS generation.
Transduction efficiencies of four mammalian cell lines were compared by detecting the expression of the reporter gene green fluorescent protein (gfp), delivered by the baculovirus genome.
In this study, we investigated the immune characters of hWJMSCs and its derived cartilage cells (hWJMSC-Cs) by detecting the expression of major histocompatibility complex I/I(MHC-I/II), costimulatory molecules (CD40, CD80 and CD86) and immune inhibitors including human leukocyte antigen G (HLA-G), indoleamine-2,3-dioxygenase (IDO), and prostaglandin E2 (PGE2).
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Semi-quantitative PCR was used first to detect the expression of testis/ovary specifically expressed genes.
We further used RT-PCR to detect the expression of TNF-h (Fig. 3B).
Allele-specific real-time PCR was employed to detect the expression of H3mm753.
So we detected the expression of ATG5 and ATG7 through silencing or over-expression of miR-3185 in HTR8 cells.
Subsequently, we also detected the expression of hLF at the protein level by SDS-PAGE and western blot analyses.
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