Single clones were analyzed by digestion with restriction endonucleases to detect length polymorphisms, which might be indicative of alternative mRNA isoforms.
The AFLP maker has been widely used to detect length changes of restricted DNA fragments subsequent to PCR amplifications [ 13].
We aimed at discovering insertion/deletion mutations through electrophoretically detected length polymorphisms in PCR products amplified from the Hsp83 gene and its flanking regions.
Here, the authors detected length ranges within 40 100 nm by utilizing information from electron microscopy about the ultrastructural organization of actin and myosin filaments within the sarcomere repeat.
Today, the majority of forensic DNA tests employ PCR and capillary electrophoresis (CE -based fragment analysis methods to deteCE -based variation in short tandem repeat (STR) markers.
Although Algorithm 2 has no explicit notion of a maximal bubble length, it may implicitly detect length limitations for both bubbles and helical regions by the absence of the "next bigger goat".
In the view of analytical technique, a standard PCR assay using primers that straddle the ITD mutation has been developed to detect length-altering mutations of ITD.
Using this scheme, a feature extraction method is proposed for detecting the length of a line segment.
IGFBP-3 processing was examined with polyclonal antibodies detecting full-length form and fragments of IGFBP-3 (Santa Cruz Biotechnologies, CA).
The eukaryotic promoters are usually identified by detecting full-length cDNA, e.g., oligo-capping [ 8].
The primer pairs for var genes 5, 11, 13, 27, 28, 29, 31 and 35 were thus verified as specific for detecting full-length transcripts.
