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The copper nanoparticles in the parts under study were detected with the method of muriatic benzidine and ammonium thiocyanate, the presence of iron being detected through a specific reaction, Perls' reaction [ 12].
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Only one or two of these 82 cases would have been detected with the methods used till the end of 2012 in the Cologne laboratory.
However, as long as the proportion of birds detected with the intensive method remains constant over time, the DS method will provide an unbiased estimate of population trend.
We used the proportion test to analyze differences in the number of episodes of HICP detected with the two methods and the paired t test to analyze differences in the percentage of time of HICP.
We suppose that positive results of PCR tests, especially in patients with non-specific symptoms in an early phase of infection, when antibodies cannot be detected with the help of traditional methods, may be helpful in the diagnosis and introduction of treatment of zoonoses such as Lyme disease (LD), tick-borne encephalitis (TBE), anaplasmosis or babesiosis.
No change in sensitivity was detected with the introduction of a 96-well extraction method.
Protein signals were detected with the ECL-plus method (GE Healthcare) followed by scanning of the blots, or, in case of Cy5 incubation, the blots were directly scanned with a Typhoon 9400 imager (GE Healthcare).
Only for one SNP on the OXTR gene (rs59190448) was a sign of positive directional selection detected with all three methods of selection detection.
However, over 700 probe sets were only detected with two of the methods and both Methods 2 (Globin PNAs_Affymetrix) and 4 (no depletion_NuGEN) uniquely detected a few hundred additional transcripts.
The differences in the number of genes with evidence of recombination detected with each method are related to (i) the power of the methods to detect recombination in sequences with different divergence and recombination levels, as well as (ii) the number of false positives associated with each method under different scenarios of heterogeneous substitution rates and convergent evolution.
Selection was based on relatively high levels of expression that could be detected with this method and because of potential biological interest.
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