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Significant differences in the setting time, cement hydration rate, hydration heat and the content of Ca(OH 2 are detected utilizing different types of emulsifier.
Standard curves for quantitation of TF levels within the homogenates were generated by spiking known quantities of purified recombinant mouse TF (R&D systems) into each well which was captured utilizing a rat monoclonal anti-TF antibody (generously provided by Daniel Kirchofer, Genentech, San Francisco, CA) and detected utilizing a goat polyclonal anti-TF antibody (R&D systems).
We labeled the receptors remaining at the plasma membrane after ligand stimulation with anti-FLAG-Cy3 antibodies prior to cell permeabilization, whilst total receptor in the cell was detected utilizing anti-FLAG-FITC antibodies after ligand addition and cell permeabilization.
Similarly, eNOS was detected utilizing a monoclonal antibody purchased from BD Biosciences (San Jose, California).
Bound antibodies were detected utilizing the mouse EnVision+, HRP kit (Dako).
CA72-4 was detected utilizing a solid phase two-site immunoradiometric ELSA-CA72-4 assay (Cisbio Bioassays, France).
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These coexpression products were detected by utilizing SDS-PAGE and confirmed by immunoblotting.
The defects between third layer and fourth layer in riveted structures can be detected by utilizing the two-stage differential coil probe.
Actin1 conservation was detected by utilizing 18S ribosomal RNA (GenBank AB253775) as our control.
Joachim et al. [11] detects obstacles utilizing a model of human color vision.
Then, the potential lesion voxels are detected and utilized as the initial seed points for region-growing algorithm.
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