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As the visible components of cell culture medium were mainly composed of exosomes, we detected the level of 14-3-3ζ 14-3-3ζ 14-3-3ζlated respectinexosomes the serum of healthy isolatedals, serespectivelyatients, cell culture medium ofromCC97H cells (expressing relathee higher level of 14-3-3ζ), Lv-serum transfected MHCC97H cells (Fig. 6d), SMCC7721 cells and Lv-14-3-3ζ transfected SMCC7721 cells.
In this case CEUS overcame colour Doppler limitations and detected the level of occlusion.
Next, we detected the level of lipogenesis-related proteins including C/EBPβ, C/EBPα, PPARγ and FABP4 [18, 21].
As free fatty acid (FFA) and glycerol are the degradation products of TG, we detected the level of FFA and glycerol in the medium.
We detected the level of endogenous PKCα and the activated form of PKCα, phospho-PKCα, in HepG2 cells using Western blot analysis.
To verify the microarray results and to improve our hypothesis, we analyzed the expression profiles of genes OsWRKY14, AS, IGPS, TS and TDC by real-time PCR (Figure 1), and detected the level of tryptophan and serotonin by High-performance liquid chromatography (HPLC) (Figure 2), in the leaves of WT, spl5-NL (No lesion), spl5-FL and spl5-ML, respectively.
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Moreover, apocynin also prevented the activation of NLRP1 inflammasome as detected the levels of cleaved caspase-1.
When infectious virus was detected, the levels were always <10% of the values of latent virus.
Moreover, we detected the levels of cell cycle regulatory proteins.
XL, SX, QZ and DW detected the levels of arsenic speciation in the urine.
However, although sufficiently high to be detected, the levels of Chk1 phosphorylation were not sufficient to inhibit DNA replication.
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