Subsequently, we also detected the expression of hLF at the protein level by SDS-PAGE and western blot analyses.
RT-PCR detected the expression of mRNAs for UT and AT1 receptors.
We also detected the expression of p62 to confirm the autophagic flux, however, no significant changes were found (Supplementary Figure S2).
To investigate the effects of OMT on autophagy in SW982 cells, we detected the expression of type II LC3 (LC3-II), autophagyagy marker, by western blotting.
To investigate the relationship between HMGB1 and autophagy, we first detected the expression of HMGB1 in SW982 cells treated with OMT.
We detected the expression of 30 of these targets and describe the preparative purification of two arbitrarily selected proteins.
To investigate whether miR-200c-3p miR-200c-3p miR-200c-3pneumonis involvedn, we collected plasma from 56 severe pneumonin patientsevere21 healthy volunteers and then detected the expneumoniaof minfectionp in the plasma.
To verify our results, we detected the expression of SOX-2, OCT-4, KLF-4, Nanog and Lin-28A under hypoxia and found that hypoxia promoted the expression of these factors remarkably.
She found significant evidence for alternative splicing in tomato because she detected the expression of 90,000 genes in tomato (about four-fold more than predicted from analysis of the genome).
We detected the expression of mir-122 across five mouse tissues and the result was consistent with that TaqMan microRNA assay.
We also detected the expression of EGFR downstream signaling proteins.
