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In our database searches we detected plant MBD proteins as well.
The public datasets where miR168 sequence is over-represented in detected plant miRNAs are from insects, chicken and mammals.
The female index was calculated as follows: FI = (number of cysts and females on detected plant)/ average number of cysts and females on 'Lee 68') × 100.
Unlike publically available datasets, the predominant detected plant miRNA for most insect libraries sequenced, including corn-fed insect libraries, is miR1507, which is not found in monocots such as corn.
The index as used by Golden et al. (1970) was calculated as follows: FI = (Number of cysts and females on detected plant)/(Average number of cysts and females on 'Lee68') × 100.
a 07-03: Rinoculatedated with the SCN race 3 in 2007; b 07-14: Rinoculatedated with the SCN race 14 in 2007; c 08-03: Rinoculatedated with the SCN race 3 in 2008; d 07-03: Rinoculatedated with the SCN race 14 in 2008; FI = (Number of cysts and females on detected plant)/(Average number of cysts and females on 'Lee68') × 100.
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Although 13 of the 15 the kanamycin-resistant transgenic plants test positive for the RNAi construct in PCR, some detected plants showed nonspecific bands.
Microscopic examination of pollens detected plants with a broad taxonomic range covering 26 families.
In case of small populations (generally < 100 individuals) all detected plants were sampled.
Table 3 Decreased pollen viability in transgenic plants of torenia selected by kanamycin resistance Genotype Pollen viability Number of detected plants Variation frequency of pollen viability R0 88.3 ± 2.7a 3 0 Transgenic plants 53.5 ± 24.3b 8 75.0 Data are expressed as mean ± SD.
A quarter of the detected plant-G-protein interactors were annotated as abiotic stimulus responsive proteins, and half are annotated as metabolism.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com