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After constructing receiver operating characteristic curves to establish the optimal threshold concentration of matrix metalloproteinase-8 for a positive test result, we detected matrix metalloproteinase-8 in 27 of 30 women with intra-amniotic infection; only 10 of 47 control specimens contained matrix metalloproteinase-8 (P <.001; odds ratio, 33.3; 95% CI, 8.4, 132.7).
Maeda and Sobel [ 34] detected matrix metalloproteinases activity, while van Horssen et al. [ 54] observed NADPH oxidase, TNF-alpha and IL-10 expression in MS microglial nodules.
Immunogold electron microscopy labeling that used an anti matrix protein goat antibody detected matrix (M) proteins on the dense tubular structures as well as on virions (C. Goldsmith et al., unpub. data).
Two matrices were used as a model for a DR4-type RE: the de novo detected matrix and the same matrix modified from the positions 7 and 8 within the spacer to resemble more the DR4-type RE known in the literature.
There are three parts of information in a normalized spectrum: peak information generated by biological sample, baseline information from matrix background noise (detected matrix molecules and fragments, dark current and detected air molecules) and random white noise from the MS instrument system.
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Inspection techniques involving a FLIR thermal camera are used to detect matrix failure.
Collagen content was quantified as total insoluble hydroxyproline on day 5. Zymography and immunoblotting were used to detect matrix metalloproteinases.
Numerous specimens with 14 different layups are tested and optical microscopy is implemented to detect matrix cracks during tensile tests.
Differentiation and proliferation were evaluated at 7, 10, 14 and 21 days post-differentiation induction using the alizarin red staining (ARS) detecting matrix calcification.
Study Design: We performed a case-control study using enzyme-linked immunosorbent assays to detect matrix metalloproteinase-8 in 77 amniotic fluid specimens that were obtained by amniocentesis from women with preterm contractions or preterm labor and intact fetal membranes (n = 66) and from women with preterm premature rupture of membranes (n = 11).
Three duplex real-time reverse transcriptase-PCR assays were run on each specimen to detect matrix gene targets specific for influenza A and influenza B, hemaaglutinin gene targets specific for influenza A subtypes H1 (seasonal), H1 (pandemic) and H3, and MS2 RNA coliphage (MS2) to monitor the efficiency of the assay [S. Williams, G. Chidlow, D.W. Smith, manuscript submitted].
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