Single clones were analyzed by digestion with restriction endonucleases to detect length polymorphisms, which might be indicative of alternative mRNA isoforms.
The AFLP maker has been widely used to detect length changes of restricted DNA fragments subsequent to PCR amplifications [ 13].
Today, the majority of forensic DNA tests employ PCR and capillary electrophoresis (CE -based fragment analysis methods to deteCE -based variation in short tandem repeat (STR) markers.
Although Algorithm 2 has no explicit notion of a maximal bubble length, it may implicitly detect length limitations for both bubbles and helical regions by the absence of the "next bigger goat".
In the view of analytical technique, a standard PCR assay using primers that straddle the ITD mutation has been developed to detect length-altering mutations of ITD.
Different bond types can be distinguished by considering detected line length, width and arrangement patterns.
Western blot analysis of the purified proteins using anti-MtbMfd polyclonal antibodies detected full length MtbMfd as well as its variants (Fig. 3C).
In cases where no ligation products were detected, HPT length was determined by sequencing.
However, no significant correlation was detected between length and condition factor.
