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The BLV tax gene was detected as suggested by the manufacturer, using the Cycleave PCR BLV detection kit (TaKaRa Bio Inc ., which amplified the BLV tax gene and detected it with the FAM-labeled Cycleave probe.
The algorithm detected IT with high sensitivity and specificity in 14 ventilated patients.
When a variant was present at low frequency (< 15%), the Illumina MiSeq detected it with significantly higher frequency than the Ion Torrent PGM.
We did not sample the allele LEAFY-4 directly by cloning analysis in the individuals H21, 194 and 378, but we detected it with the help of PCR using LEAFY-4 -specific primers (data not shown).
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Mr. Speis said he was unable to hear the hum or detect it with his equipment.
The ability to detect it with brain scans of living people is at least a couple of years off.
The possibility to reliably detect it with TCS would provide a low-cost, harmless, widely available tool.
The microparticles can be used in immunoassays as the antibody-coated solid phase to capture an antigen and then detect it with a fluorescently labeled tracer antibody.
Everybody is used to the hype that surrounds Apple's events; it's so heavy you can detect it with a Geiger counter and you have to be sanitized before you leave the auditorium.
For now, they don't have a clue about the virus's origins or why it's suddenly causing an outbreak; in order to speed up the process, they want to share the virus and protocols for detecting it with anyone interested in studying the disease or developing diagnostic tools and vaccines.
Double-labeling of nerves and taste buds in the same specimen was accomplished by first applying the anti-calretinin and detecting it with red, followed by an overnight wash, and then applying the anti-acetylated tubulin and detecting it with green (e.g., goat anti-mouse FITC).
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