After data cleaning, no recombinant variants were detected in the 10,000 template mixture while two recombinant variants were detected in the 100,000 template mixture at proportions of 0.22% and 0.18% of the total number of sequences, respectively.
The threshold cycle, or C T value, is the cycle at which a significant increase in normalized reporter fluorescence, ?Rn, is first detected (See Appendix 3); where ?Rn is calculated from Rn + and Rn -, where Rn + is the Rn value of a reaction containing all components, and Rn - is the Rn value of an un-reacted sample (the baseline value or the value detected in the no-template control, NTC).
Positive signal was detected in the reactions containing RNA template derived from the SARS-CoV TW1wanese strain TW1 but not in those from 12 healthy controls and from two human coronaviruses (229E strain and human enteric coronavirus Dallas 1 strain) and not in the reaction containing no RNA (data not shown) (19, 22 ).
Normal 2D are defined by having fewer events detected in the complement strand than in the template strand.
For each vehicle detected in the first image, a circular template image of a certain radius (e.g. r = 3 m for cars) is generated at the position of the vehicle detection in the first image.
To properly correct the spatial anomaly detected in the DNA, the nascent and template DNA strands have to be distinguished.
The size of the product amplified using the 5p primer was consistent with presence of the small RNA template detected in the sequencing rather than full length hY4 RNA.
Finally, Tau template sequences were detected in the novel open box sessions significantly more often than WT templates.
As shown in Figure 4A, transcripts of TRV1 and TRV2 were detected, through RT-PCR, in the small fruit of the infiltrated plant 1 but not in the small fruit of the infiltrated plant 3, while no amplification products were detected in the "NO reverse transcriptase" and "NO template" negative controls.
Interestingly, we found that generation of ssDNA oligos from chromosomal DSBs is entirely dependent upon MRN, whereas small amounts of ssDNA oligos generated from synthetic DNA templates can still be detected in the absence of MRN (data not shown).
