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Twenty-eight 16S rRNA gene-containing archaeal clones and sixteen bacterial clones were detected in the library.
Notably, Helicobacter was only detected in the library constructed from MD 257 but there were 12 unique transcript-tags assigned to this genus.
The sample size N = Σx⋅fx and s = Σ fx is the total number of unique transcripts detected in the library (x≥1).
Furthermore, we noticed that the highest number of miRNAs, both in sorts and quantities, were detected in the library made from RNA isolated at molting larva stage (MLS 33 out of 77 sequences, representing 12 different miRNAs.
N, the homologous UniGene cluster was not detected in the library.
Seven orthobunyavirus sequences were detected in the library prepared from pooled RNA from 3 animals of 1 farm (BH 80/11, Table).
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Proteolytic enzymes were among the most abundant sequences detected in the libraries.
Most of the reference miRNAs for these less conserved miRNA family variants were poorly or not detected in the libraries.
Only novel mature and mature-star miRNA sequences detected in the libraries were used for target prediction analysis.
MiR2118 was not detected in the libraries derived from seedlings, root apices and leaves, but could be detected in libraries derived from early development stages of maize anthers [ 34, 35].
This was also confirmed in this study as we revealed that for genes with few sequences (total n = 1-5) detected in the libraries, the expression profile from oocyte until gastrula could not be reflected in expression profiles measured with quantitative PCR (qPCR) for the same genes.
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