Sentence examples for detected in the flow through from inspiring English sources

On the other hand, a single polypeptide of 22 kDa was detected in the flow through fraction of the G1485A mutant (Figure 4B, lane 2).

The receptor was completely captured by the bead matrix, as no hOR17-4 was detected in the flow through by western blot.

On the blot, RhCG could not be detected in the flow through (FT), demonstrating that amount of immobilized anti HA was adequate to capture all extracted protein present in the lysate.

Any activity detected in the flow through during IMAC purification was ascribed to endogeneous E. coli NADH quinone oxidoreductases.

After the column was washed with five column volumes of buffer A, the majority of the EngPA activity was detected in the flow through.

Moreover significant amounts of the recombinant protein were detected in the flow through (FT, Fig. 5B), indicating that there is still room for optimization of the purification procedure.

The beads were further washed using a buffer containing 10 mmol/L imidazole until no traces of the protein were detected in the flow-through.

The column was washed with 50 mM Tris at pH 8, 500 mM NaCl buffer supplemented with 10 mM imidazole until no protein was detected in the flow-through.

After adjusting salt concentration to 3 M, the eluate was subjected to Phenyl Sepharose CL-4B chromatography employing a reverse NaCl-gradient (2→0 M) in buffer A, and nuclease activity was detected in the flow-through and washing fractions only.

The RNA was hardly detected in the flow-through after being pulled down with LAMP2C peptide (Fig. 2D), indicating that LAMP2C peptide bound to almost all RNAs.

Mass spectrometric analysis revealed that the eluted IC fraction from RA SF contained mainly immunoglobulins, while almost none were detected in the flow-through fraction (data not shown).