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All the reaction mixtures were detected in duplicate, and at least three independent assays were performed for each sample.
Each serum was detected in duplicate.
PrPSc signals were detected in duplicate samples from cow 5468 after three rounds of amplification (Figure 7C) but were not detected in samples from cow 5499 (Figure 7B), as confirmed in samples from cow 5550 (Figure 6A).
Each sample was detected in duplicate repeat.
Pesticide residues were not detected in duplicate beverage samples.
Thus, the overall percentage of unigenes detected in duplicate is low for Cleome.
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Positive selection could not be detected in other duplicate gene groups.
PrPSc signals were also detected in both duplicate samples treated at 160°C and 180°C for 3 h after two or three rounds of amplification.
Hence, when the outgroup was excluded, positive selection was detected in 21 duplicate loci; Table 3 describes the results for 16 loci that included positively-selected loci both with and without an outgroup comparison (these are also marked with red arrows in Additional file 1).
Samples were considered positively amplified when a comparative threshold cycle (CT) <50 was <span class="lh">detected in both duplicates, with <1 CT variance between duplicates.
§ Calculated for all assays detected in both duplicates.
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