Thus, our de novo transcriptome captures a large fraction of genes present on the neo-sex chromosomes, with 1,754 protein-coding genes being expressed from both the neo-X and neo-Y, 387 transcripts only being detected from the neo-X, and 109 transcripts only from the neo-Y.
These values were then normalised against the quantities of transcripts detected from the reference gene actin.
The only transcripts detected from the novel wheat paralogs related to TaGA2ox6 were found in mature leaves, from TaGA2ox-B12 (Fig. 5C and Additional file 3).
Microarray results from three of these samples are listed in Additional file 3. The numbers of gene transcripts detected from the three single-cell samples were 590, 576, and 614, respectively.
Early transcripts were detected from the entire genome with the exception of a fragment at 0.278-0.305 map units.
Transcripts were detected from the majority of candidates using DGE and qRT-PCR, suggesting that most genes, including those encoding the novel bZIPs, are not pseudogenes.
RNA transcripts were detected from the opposite strand of the same gene, referred to as PEG11AS (formerly known as antiPEG11) [ 3].
As shown, 660 (58.2%), 663 (58.4%), and 662 (58.3%) gene transcripts were detected from the three 100-cell samples of NCI/ADR-RES, respectively.
No transcripts were detected from the recessive allele of either vrn-A1 or vrn-B1 in the winter DH lines in leaves at the third, seventh, or ninth leaf stages.
As shown in Table 1, 630 (96.7%) of the 650 gene transcripts detected from all the 100-cell samples were also detected from the 10,000-cell sample.
