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In addition, we detected expression of the cholinergic enzymes choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) (Figure 1).
We detected expression of the GPI1 protein in various tissues and germ cells isolated from mouse testis.
In addition, we also detected expression of the α-secretase ADAM10 and C83, a product of APP cleavage by α-secretase, in the brains of these three groups.
We also detected expression of the neuroendocrine marker secretogranin II, which is often observed in SCLC, in the orthotopic tumor (Fig. S1c, d).
Thus, the number of transcripts per million (TPM) was set to 2 for tissues with no detected expression of the gene in question.
A recent transcriptome work using P. acnes[ 48] detected expression of the whole respiratory chain under anaerobic growth conditions, including NADH dehydrogenase/complex I (NDH-1) and succinate dehydrogenase/fumarate reductase (SdhABC).
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However, at the exposure times used to detect expression of the transgene, no endogenous cyclin C mRNA expression was detectable (data not shown).
We were unable to detect expression of the entire Glp1r coding sequence in macrophages isolated from ApoE, C57BL/6, and IL10 mice.
In addition, we could detect expression of the endodermal regulators Sox17, Gata6 and FoxA2 [31], [32], [33], [33].
The primers used to detect expression of the corresponding genes are described in Table 2. Murine Slfn4 cDNA was cloned into the pGene vector (Invitrogen, Carlsbad, CA, USA).
Surprisingly, we could not detect expression of the important complement inhibitor SCIN in the nose although it was shown that nasal secretions contain complement proteins [37].
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