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A gene is considered expressed if the detected expression level is above a certain threshold.
In all of the comparative analyses, only the positively detected expression data were used.
In the discovery phase, we used the TaqMan OpenArray Human MicroRNA Panel Applied Biosystemss) according to the manufacturer's instructions and detected expression of 754 human miRNA transcripts.
However, we also detected expression of E-cadherin in a number of subepithelial cells in and around fibrotic areas (Figure 6B + C).
Furthermore, we detected expression of several grain size genes involved in the regulation of cell proliferation in spikelet hulls, including GW2, GS3 and GL3.
Our results, however, found no significant alterations in gene copy number for HER2, although IHC staining detected expression of HER2 oncoprotein in 33% of the 45 NPC tumors.
In both samples we detected expression of all Prdm5 16 genes (data not shown).
Several hypotheses can explain the difference in detected expression between whole bodies and testes alone.
We detected expression of Sprouty4 or Lfng in E10.5 mouse PSM by BCIP/NBT.
We detected expression of Hcp at OD600 1.0 and the highest expression was observed at OD600 2.0 (Fig. 1).
Using an HA-antibody, we detected expression of CagA-HA throughout the differentiated eye epithelium (Fig. 1C).
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