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When binding affinity to a transition-state analog was detected, evolution of the catalytic antibody was carried out in vitro on yeast-cell surface.
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Several solutions have been developed to deal with this issue, but they often focus their monitoring on a single aspect of the collaboration and/or impose a single adaptation strategy to face the detected evolutions.
How do researchers detect evolution within a single HIV infection?
Detecting evolution in wild host populations involves techniques and tools founded on these four principles.
Whole-genome scans for positive Darwinian selection are widely used to detect evolution of genome novelty.
The described flow cytometry panel could be used for serial monitoring during follow-up to detect evolution of GPI-AP deficient populations and to assess therapy effects.
To detect evolution of constitutive upregulation of glycolysis, we stained specimens for GLUT-1 since prior studies have demonstrated a direct correlation between GLUT-1 expression and increased glucose flux as measured by FdG PET (Brown et al, 1996, 2002).
However, although they detected NO evolution for the first event they did not detect NO for the second event and assigned it to reduction of CoO, whereas we observe significant NO/NO2 evolution as well as water evolution for both events.
We detected the evolution of superoxide by virtue of its ability to react with the water-soluble derivative of tetrazolium, WST-1 [ 2- 4-iodophenyl -3- 4-nitrophenyl -5- 2,4disulfophenyl -2H-tetrazolium].
Again, no amelioration of the CA-125 levels was noted, and imaging detected disease evolution.
The alternative interpretation is that PSI-BLAST detected convergent evolution, i.e. the acquisition of common structural elements in otherwise disparate factors.
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