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By subsequent analyses of off-target disruption within the CCR2 locus of the same cells, we detected disruption (3%) induced by plasmid-encoded ZFNs, whereas indels and mismatches were not evident after ZFN protein transduction.
42 Obatoclax was developed from a lead compound identified in a functional screen of natural compounds that detected disruption of protein protein interactions between members of the BCL-2 family.
In addition, we performed immunoprecipitation of p53 and FAK in untreated and R2-treated HCT116p53+/+ xenografts and detected disruption of FAK and p53 complex in the HCT116 p53+/+ xenografts.
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However, we also detected disruptions of ZO-1 after MCAO in the cortical MCA territory of the guinea pig whole brain preparation which resembles more closely the in vivo situation.
Even if OCTA does not possess the capability to detect disruption of the RPE secondary to active inflammation, it may provide other meaningful information.
When the network layer detects disruption in the route due to mobility, then it informs the source using a route failure notification (RFN) message.
Furthermore, EBER1 expression in these mice is low and very high levels of EBER1 may be required to detect disruption of PKR activity.
Although we did not detect disruption of ciliogenesis, primary cilia are the site for other important cellular functions, including Hedgehog signaling and trafficking of intraflagellar transport molecules.
Our Foxa2 mutant screen was performed at E7.5, which we expect is too early to detect disruption of the structural genes expressed later in differentiation of mature notochord.
Onset, offset, and duration of iSP were determined using an adjusted version of the Teager Kaiser Energy Operator (Solnik et al. 2010), detecting disruption in the ongoing EMG activity.
In general, these results confirmed that USVs emitted during such social interaction can be used as an indicator of social recognition, and therefore as a dependent variable to detect disruption of social memory in mice.
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